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Our Lab

Our lab is situated in the Department of Biochemistry at the ÒÁÈËÖ±²¥. We are interested in characterizing life processes at the atomic level using multi-nuclear (non-radioactive ¹H, ¹³C, and ¹⁵N) multi-dimensional solution NMR spectroscopy, along with other biophysical techniques. We have a number of ongoing projects, ranging from the development of solution NMR methodology to protein structure determination to clinical application.

Our Lab Publications

• Zahran, S., Figueiredo, V. P., Graham, M. M., Schulz, R. & Hwang, P. M. Proteolytic Digestion of Serum Cardiac Troponin I as Marker of Ischemic Severity. The Journal of Applied Laboratory Medicine jalm.2017.025254 (2018). doi:10.1373/jalm.2017.025254 [ahead of print]
• Mahmud, Z. & Hwang, P. M. Cardiac Troponin Complex: Cardiac Troponin C (TNNC1), Cardiac Troponin I (TNNI3), and Cardiac Troponin T (TNNT2). Encyclopedia of Signaling Molecules. (2017)
• Danmaliki, G. I., Liu, P. B. & Hwang, P. M. Stereoselective Deuteration in Aspartate, Asparagine, Lysine, and Methionine Amino Acid Residues Using Fumarate as a Carbon Source for Escherichia coli in D2O. Biochemistry 56, 6015-6029 (2017).
• Cai, F., Li, M.X., Pineda-Sanabria, S.E., Gelozia, S., Lindert, S., West, F., Sykes, B.D., and Hwang, P.M. Structures reveal details of small molecule binding to cardiac troponin. J. Mol. Cell. Cardiol. 101, 134-144 (2016).
• Hwang, P.M. "(De-)sensitization" vs. "Uncoupling": what drives cardiomyopathies in the thin filament? Cardiovasc. Res. 109, 185-186. (2016).
• Li, M. X. & Hwang, P. M. Structure and function of cardiac troponin C (TNNC1): Implications for heart failure, cardiomyopathies, and troponin modulating drugs. Gene 571, 153-166 (2015).
• Hwang, P. M. & Sykes, B. D. Targeting the sarcomere to correct muscle function. Nat Rev Drug Discov 14, 313-328 (2015).
• Zahran, S., Pan, J. S., Liu, P. B. & Hwang, P. M. Combining a PagP fusion protein system with nickel ion-catalyzed cleavage to produce intrinsically disordered proteins in E. coli. Protein Expr. Purif. 116, 133-138 (2015).

Lab Members